Figure 1.

Induction of HA2X phosphorylation (γ-H2AX) during DNA damage. Tca8113 cells were cultured and exposed to DOX (0.2 μmol/L and 0.5 μmol/L) for 9 h, or treated by γ-ray (2 Gy and 8 Gy) for 1 h. After harvest, the cells were fixed, permeabilized, and immunolabeled with anti-γ-H2AX antibody and secondary antibody (green). The nuclei were stained with DAPI (blue).