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. 2016 Nov 15;8(11):4750–4763.

Figure 6.

Figure 6

LY2405319 inhibits liver fibrosis in MCD diet-fed ob/ob mice. (A) Representative photomicrographs showing the effect of the MCD diet on collagen deposition in MCD-vehicle-fed and MCD-LY2405319-fed ob/ob mice. Paraffin-embedded sections from MCD-vehicle-fed ob/ob and MCD-LY2405319-fed ob/ob mice were stained with Masson’s trichrome stain. The arrows indicate pericellular fibrosis in MCD-vehicle-fed ob/ob mice (n = 10 per group). (B) Analysis of collagen type-1α and TGF-β1 mRNA expression by qRT-PCR. **P < 0.01 (C) Serum AST and ALT levels in MCD-vehicle-fed or MCD-LY2405319-fed ob/ob mice. **P < 0.01 Vehicle vs. LY2405319 (n = 6-8 per group). (D)Hepatic TG and cholesterol levels in MCD-vehicle-fed or MCD-LY2405319-fed ob/ob mice (n = 6-8 per group). *P < 0.05, **P < 0.01. (E) Effect of LY2405319 on inflammatory signaling in the livers of the MCD diet-fed ob/ob mice treated with or without LY2405319. Western blot analyses of liver extracts were performed to detect NF-kB p65, p38 and JNK 1/2 phosphorylation. GAPDH was used as a loading control. The blots are representative of three independent experiments.