Figure 4.

Knock down of AKIP1 inhibits the migration and invasion capacities of breast cancer cells. A. The transfection efficiency of AKIP1 was analyzed by measuring transcript levels using qRT-PCR analyses in MCF-7 and SK-BR-3 cells. B. Effect of AKIP1 knockdown in cell migration of MCF-7 and SK-BR-3 was determined by wound healing assay (left panels), the uncovered areas in the wound healing assays were quantified as a percentage of the original wound area (right panels). C. Transwell cell migration assay and Matrigel invasion assay of breast cancer cells transfected with Si-Control, AKIP1-Si#1, and AKIP1-Si#2 (left panels). Quantifications of migrated cells through the membrane and invaded cells through Matrigel of each cell line are shown as proportions of their vector controls (right panels). Bar graphs show the statistical analysis of three independent experiments (*P<0.05).