FIG 1.
Monoclonal antibody (MAb) reactivity against various V1V2 scaffolds. The optical densities (ODs) of the ELISA reactions between several human MAbs (used at dilutions of 10 μg/ml) and various V1V2-scaffold antigens are shown. MAbs are designated specific for the V2i, V2p, or V2q epitope, as defined in the text. Diagrams of V1V2A244-tags, of V1V2ZM109-1FD6, and of cV292TH023, which were used as antigens in these ELISAs, are shown. For V1V2ZM109-1FD6, V1V2 is shown in purple, and 1FD6 is in gray. The four strands, A, B, C, and D, of V1V2 are marked. Negative controls consisted of human MAb 1418, specific for parvovirus B19, and human MAb 2299, specific for gp70 of murine leukemia virus.