FIG 4.

Competition of V1V2 MAbs against each other. (A) An illustration of the competition assay setup. (B) To establish the baseline, we first competed each V1V2 MAb against itself. Shown here is V2q MAb PG9 competed with itself for structurally constrained antigen V1V2(ZM109)-1FD6 coated on the plate using PBS as a negative control. (C to F) Competition of V2q MAb PG9, V2i MAbs 697-D and 830A, and V2p MAb CH58 by biotinylated MAbs PG9 (C), 697-D (E), and 830A (F) for V1V2(ZM109)-1FD6 coated on the plates. Panel D shows competition of the same MAbs by biotinylated V2p MAb CH58 for V1V2(1086)-tags. Note that PG9 and CH58 each only competed with itself (C and D), while V2i MAb 830A and 697-D competed with each other. Although CH58 does not bind V1V2(ZM109)-1FD6 and PG9 does not bind V1V2(1086)-tags, they were included for references.