FIG 1.

Ectopically expressed IFIT3 had no effect on the replication of WT HSV-1. (A and B) HEK293T cells were transfected with vector or IFIT3-Myc plasmid. At 8 h posttransfection, the cells were infected with WT HSV-1 at an MOI of 0.5, and then cells were harvested at the indicated time points postinfection and subjected to WB analysis with antibodies against UL46, VP22, Myc, or β-actin (A) or viral plaque assay on Vero cells (B). (C) HEK293T cells were transfected with vector or IFIT3-Myc plasmid. At 8 h posttransfection, the cells were infected with VSV at an MOI of 0.2, and then cells were harvested at the indicated time points postinfection and subjected to viral plaque assay on Vero cells. The data represent results from one of the triplicate experiments. Error bars represent mean standard deviations (SD) from three independent experiments. Statistical analysis was performed using Student's t test. *, 0.01< P < 0.05; **, 0.001 < P < 0.01.