TABLE 2.
Primers used for construction and site-directed mutagenesis of HIV-2 envelope
| Primer ID | Gene | Sequencea (5′-3′) |
|---|---|---|
| F3 | vpr | 5′-TAGACATGGAGACACCCTTGAARGMGC-3′ |
| A1m2F | rev | 5′-GCGCTCTAGAGCCACCATGAACGAAAGGGCAGACGAAGAAGGACTCC-3′ |
| R1 | nef | 5′-TGTAAWACAKCCCTTCCAGTCCYCC-3′ |
| NT5mR | env | 5′-CYTCACAGGAGGGCRAKTTCTGC-3′ |
| ROD10/14-XbaI-F | env | 5′-GCGCTCTAGAATGAACGAAAGGGC-3′ |
| ROD10/14-XhoI-HAtag-R | env | 5′-GCGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATACAGGAGGGCGCT-3′ |
| HIV-2 Rev-XbaI-F | env | 5′-GCGCTCTAGAGCCACCATGAACGAAAGGGCAGACGAAGAAGGACTCC-3′ |
| HIV-2 Rev-NheI-F | env | 5′-GCGCGCTAGCGCCACCATGAACGAAAGGGCAGACGAAGAAGGACTCC-3′ |
| 1-SalI-HAtag-R | env | 5′-CGCGTCGACTCAGGCGTAGTCAGGCACGTCGTAAGGATACAGGAGGGCGAGTTCTGCTCC-3′ |
| 2-XhoI-HAtag-R | env | 5′-CGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATACACTATCCCGGCCAGTAAAG-3′ |
| 3-XhoI-HAtag-R | env | 5′-GCGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATACAGGAGGGCGAGTTCTGCCC-3′ |
| 3-s10-XhoI-HAtag-R | env | 5′-CGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATATGTCATATTGTCCCATTTAG-3′ |
| 3-s11-XhoI-HAtag-R | env | 5′-CGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATATTCTATCTGCCAAGGCCAGG-3′ |
| 4-XhoI-HAtag-R | env | 5′-CGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATACAGGAGGGCGAGTTCTGCTTC-3′ |
| 5/6-SalI-HAtag-R | env | 5′-CGCGTCGACTCAGGCGTAGTCAGGCACGTCGTAAGGATACAGGAGGGCGATTTCTGCTCC-3′ |
| 7-XhoI-HAtag-R | env | 5′-GCGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATACAGGAGGGCGAGTTCTGCCC-3′ |
| 8-s3-XhoI-HAtag-R | env | 5′-CGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATATTCCTTCTCTGTCTGGCTGT-3′ |
| 8-s4-XhoI-HAtag-R | env | 5′-CGCCTCGAGTCAGGCGTAGTCAGGCACGTCGTAAGGATATCGGCCAAGGCCAGGAGCTG-3′ |
| m1-F | env | 5′-GTGAATCACCTAAAGAAGGCACAAACACAACTAGCACACCTAGCACAGCTGTAAATGACA-3′ |
| m1-R | env | 5′-TGTCATTTACAGCTGTGCTAGGTGTGCTAGTTGTGTTTGTGCCTTCTTTAGGTGATTCAC-3′ |
| m2-F | env | 5′-GTGAATCACCTAAAGAAGGCAACACAACTAGCACACCTGTAAATGACAGT-3′ |
| m2-R | env | 5′-ACTGTCATTTACAGGTGTGCTAGTTGTGTTGCCTTCTTTAGGTGATTCAC-3′ |
| m3-F | env | 5′-GCAAAAACTAAATAGCTGGGATATTTTTGGCAACTGGTTTGACTTGACCT-3′ |
| m3-R | env | 5′-AGGTCAAGTCAAACCAGTTGCCAAAAATATCCCAGCTATTTAGTTTTTGC-3′ |
| m4-F | env | 5′-ACAGAACAGGACAAATCAGACAAAACGCAATTATGTGTC-3′ |
| m4-R | env | 5′-TTGCGTTTTGTCTGATTTGTCCTGTTCTGTACCCAATTG-3′ |
| m5-F | env | 5′-TTTACTGGCTGGGATAGTGCAGCAACAGCAACAGCTGTTG-3′ |
| m5-R | env | 5′-TGCTGTTGCTGCACTATCCCAGCCAGTAAAGTCCGGGAC-3′ |
| m6-F | env | 5′-AATTGTTAAGTAGACTTAGAAAGGGCTATAGGCCTGTTTTCTC-3′ |
| m6-R | env | 5′-TATAGCCCTTTCTAAGTCTACTTAACAATTGTACTATGTATATTAC-3′ |
| m7-F | env | 5′-AGAGAAGAAACAGAAGAAGACGTTGGAAACAGCGTTGGAGACAG-3′ |
| m7-R | env | 5′-TGTTTCCAACGTCTTCTTCTGTTTCTTCTCTGGCTGGCTG-3′ |
a
R = A or G, M = A or C, W = A or T, K = G or T, and Y = C or T.