FIG 2.

Plaque morphologies of syncytial, EGFR-retargeted HSVs. (A) Vero cells infected for 24 h with the viruses indicated above the panels were stained with wheat germ agglutinin-Alexa Fluor 594 (red) and Hoechst 33342 (blue) for detection of cell membranes and nuclei, respectively. Dotted lines indicate the margins of plaques (white, nonsyncytial plaques; yellow, syncytial plaques). (B) Vero cells infected for 24 h with the viruses indicated above the panels were incubated with the anti-gD MAb DL6 and stained with a Cy3-conjugated secondary antibody. Red, Cy3 signals; green, EGFP signals. Rescued, a rescued virus derived by replacement of the gB:R858H and gK:A40T mutations of KGNE-BhKt BAC with the respective wild-type residues.