FIG 5.

Reduced TMPRSS12 expression decreases aMPV/B replication. (A and C) aMPV/B replication kinetics in different cells with reduced TMPRSS12 were tested for up to 96 h p.i. (B and D) At 72 h p.i., the supernatant in infected cells was collected to determine titer. (E) siRNA targeting Ch_TMPRSS12 was transfected into CEF cells. At 48 h posttransfection, TMPRSS12 mRNA expression was analyzed by qRT-PCR. The average copy numbers of Ch_TMPRSS12 mRNA were reduced from 2.04 × 10³ to 1.78 × 10². (F) aMPV/B replication kinetics in CEF cells with reduced TMPRSS12 were tested for up to 96 h p.i. (G) At 72 h p.i., the supernatant in infected cells was collected to determine titer. (H and I) Mo_TMPRSS12 or Ch_TMPRSS12 was transfected into TMPRSS12 siRNA-treated cells. At 24 h posttransfection, these cells were infected by aMPV/f. At 48 h p.i., the supernatant in infected cells was collected to determine titer. **, P < 0.05; ***, P < 0.005; #, P > 0.05 (nonsignificant).