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TMPRSS12 mRNA is expressed in chicken tissues. (A) Analysis of TMPRSS12 mRNA expression by RT-PCR. Total RNA isolated from chicken tissues was used for the amplification of TMPRSS12 mRNA by RT-PCR. The results analyzed by 2% agarose gel are shown. GAPDH, glyceraldehyde-3-phosphate dehydrogenase.(B) Analysis of TMPRSS12 mRNA expression by qRT-PCR and the rate of aMPV/B isolation. RNA was prepared as described for TMPRSS12 and β-actin and then amplified by qRT-PCR.
