. 2016 Nov 15;2016:8905675. doi: 10.1155/2016/8905675

Table 1.

List of primers targeting S. Typhi-specific genes for the development of 6 PCR assays.

Target genes	Primer labels	Primer sequences (5′-3′)	Target lengths (bp)
STY0201	0201F	ATGCTTTTAAAAAACACAACATGG	1176
STY0201	0201R	TTACGGATAGGTGATTGAAAATTG	1176
STY0307	0307F	ATGAAACCTTTATTCTCAGTGC	495
STY0307	0307R	TTAGCGTAATTCCCAGAACC	495
STY0322	0322F	ATGAAATATAAAAAAATAAGAG	678
STY0322	0322R	CTATGGATTCATTTCCATTTC	678
STY0326	0326F	ATGAATACGAATAATTCACC	261
STY0326	0326R	TTACCCTCCCCATGTCAC	261
STY2020	2020F	ATGCCTGTTATGCATAATTG	429
STY2020	2020R	TTATGCTGTTAACGAGTCGTC	429
STY2021	2021F	ATGAGTTTAGCGCAGCCTAAATCC	732
STY2021	2021R	TTAGAAGTCTCCTGCCTGGAAAC	732
16S rRNA^a	16SF	CAGGCCTAACACATGCAAGTC	1362
16S rRNA^a	16SR	GGGCGGTGTGTACAAGGC	1362

^a16S ribosomal RNA gene served as internal amplification control (IAC) [24].

F represents forward primer.

R represents reverse primer.