Fig. 4. Effect of corn silk extract on mRNA expression of enzymes related to fat synthesis in the liver and adipose tissue of mice fed high-fat diet.

Total RNA was isolated using TRI-reagent, and cDNA was synthesized using 3 µg of total RNA with SuperScriptⅡ reverse transcriptase. Real-time PCR was performed using SYBR green and standard procedures to assess mRNA expression of primer in liver (A) and adipose tissue (B) (epididymal fat pad) samples obtained from each group. Applied Biosystem StepOne software v2.1 was used. Each bar represents the mean ± SD, and different letters above each bar indicate significant differences among groups at α = o.o5 as determined by Duncan's multiple range test. ACC1; acetyl-CoA carboxylase1, ACC2; acetyl-CoA carboxylase2, G6PDH; glucose-6-phosphate dehydrogenase FAS; fatty acid synthase, SCD1; stearoyl-CoA desaturase-1, SREBP-1c; sterol regulatory element binding protein1c, PDK-4; pyruvate dehydrogenase kinase, isozyme 4, GPAT-1; glycerol-3-phosphate acyltransferase-1.