Figure 1. Impaired RANKL-induced ROS production in osteoclast precursors and osteoclasts from gp91^phox−/− mice.

Osteoclast precursors were incubated with (A) PMA or (B) RANKL after preloading with isoluminol, and superoxide production was measured. Result represent the mean ± SD of 6 (A) and 3 (B) independent experiments, *p < 0.05 and **p < 0.01 (paired t-test). (C) Osteoclasts obtained from wild-type and gp91^phox−/− mice were pretreated with DCF-DA and observed under fluorescence microscope at the magnitudes designated after PMA treatment for 10 min. The fluorescent images are representative of least 3 independent experiments, each performed in triplicate.