Figure 6. Effect of H₂O₂ on NFATc1 and NF-κB expression in osteoclasts from wild-type and gp91^phox−/− mice during RANKL-induced osteoclast differentiation.

Data are expressed as the mean ± SD of 3 independent experiments, each performed in triplicate, *p < 0.05 and **p < 0.01 (paired t-test). (A,B) Differential NFATc1 expression between wild-type and gp91^phox−/− cells during osteoclast differentiation is shown by western blotting. (C) BMMs from wild-type and gp91^phox−/− mice were subjected to RANKL-induced osteoclast differentiation following treatment with or without 0.1 μM H₂O₂. After 4 (osteoclast precursor) and 8 (osteoclast) days, expression of NFATc1 and NF-κB was assessed by western blotting.