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. 2016 Nov 29;6:37427. doi: 10.1038/srep37427

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Immunofluorescence staining of CYP3A4 protein in Huh-7.5 cells on (a) the bare PEG-DA scaffold, (b) the collagen-functionalized scaffold, and (c) the fibronectin-functionalized scaffold, on days 1, 7, and 14. CYP3A4 was stained with Alexa Fluor 488 (green), F-actin was stained with Alexa Fluor 555 phalloidin (red), and cell nuclei were counterstained with DAPI (blue). High quality figures of the day 14 merged images are shown in Supplementary Fig. S1.