Table 3. Primers and probes used for the detection and quantification by vitality qPCR.

STRAIN		Primer/Probe (5′-3′)	Fragment length
Aggregatibacter actinomycetemcomitans	Forward	GAA CCT TAC CTA CTC TTG ACA TCC GAA	80 bp
	Reverse	TGC AGC ACC TGT CTC AAA GC
	Probe	AGA ACT CAG AGA TGG GTT TGT GCC TTA GGG
Fusobacterium nucleatum	Forward	GGA TTT ATT GGG CGT AAA GC	162 bp
	Reverse	GGC ATT CCT ACA AAT ATC TAC GAA
	Probe	CTC TAC ACT TGT AGT TCC G
Porphyromonas gingivalis	Forward	GCG CTC AAC GTT CAG CC	68 bp
	Reverse	CAC GAA TTC CGC CTG C
	Probe	CAC TGA ACT CAA GCC CGG CAG TTT CAA
Prevotella intermedia	Forward	CGG TCT GTT AAG CGT GTT GTG	99 bp
	Reverse	CAC CAT GAA TTC CGC ATA CG
	Probe	TGG CGG ACT TGA GTG CAC GC
Streptococcus mutans	Forward	GCC TAC AGC TCA GAG ATG CTA TTC T	114 bp
	Reverse	GCC ATA CAC CAC TCA TGA ATT GA
	Probe	TGG AAA TGA CGG TCG CCG TTA TGA A
Streptococcus sobrinus	Forward	TTC AAA GCC AAG ACC AAG CTA GT	88 bp
	Reverse	CCA GCC TGA GAT TCA GCT TGT
	Probe	CCT GCT CCA GCG ACA AAG GCA GC
Actinomyces naeslundii	Forward	TCG AAA CTC AGC AAG TAG CCG	96 bp
	Reverse	AGA GGA GGG CCA CAA AAG AAA
	Probe	GGG TAC TCT AGT CCA AAC TGG CGG ATA GCG
Streptococcus gordonii	Forward	CGG ATG ATG CTA ATC AAG TGA CC	177 bp
	Reverse	GTT AGC TGT TGG ATT GGT TGC C
	Probe	AGA ACA GTC CGC TGT TCA GAG CAA
Actinomyces viscosus	Forward	GTG AAG GAG CCA GCT TGC TGG TTC TG	155 bp
	Reverse	CGG AAC AAA CCT TTC CCA GGC
	Probe	ATG AGT GGC GAA CGG GTG AGT AAC
Streptococcus salivarius	Forward	AAC GTT GAC CTT ACG CTA GC	192 bp
	Reverse	ACC GTA ACG TGG GAA AAC TG
	Probe	GTA GCG TCA GAG TGG TTG AC
Streptococcus oralis	Forward	ACC AGC AGA TAC GAA AGA AGC AT	229 bp
	Reverse	AGG TTC GGG CAA GCG ATC TTT CT
	Probe	AAG GCT GCT GTT GCT GAA GAA GT
Streptococcus mitis	Forward	GGC TCG TAG TCT GGA GAT GG	133 bp
	Reverse	TAG GTC GTC GTC CCA AGG AA
	Probe	CGA AGA GCA CCA ATA GCA CCT CCC
Streptococcus sanguinis	Forward	CAA AAT TGT TGC AAA TCC AAA GG	75 bp
	Reverse	GCT ATC GCT CCC TGT CTT TGA
	Probe	AAA GAA AGA TCG CTT GCC AGA ACC GG
Veillonella parvula	Forward	GAC GAA AGT CTG ACG GAG CA	171 bp
	Reverse	TGC CAC CTA CGT ATT ACC GC
	Probe	AGC TCT GTT AAT CGG GAC GAA AGG C