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. 2016 Nov 29;6:37843. doi: 10.1038/srep37843

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Phylogenetic trees were generated by using the neighbor-joining method and bootstrapped with 1,000 replicates using MEGA6 software version 6.05. Phylogenetic trees were based on the comparison of nucleotide sequences of the H4 avian influenza viruses (AIVs) isolated in this study to reference AIV sequences published in GenBank. Isolates are highlighted in red. The scale bar represents the distance unit between sequence pairs.