Figure 3. In vitro PTB siRNA decreased the proliferation of keloid fibroblasts with alternative splicing switching of genes involved in the cell proliferation.

(a) Keloid fibroblasts were transfected with PTB siRNA or control siRNA, and the expression of PTB was analysed by immunofluorescence, real-time PCR and western blot post transfection. Data are shown as the mean ± SEM, n = 4. ***P < 0.001 by paired student’s t-test. (b) Proliferation of keloid fibroblasts after PTB knockdown was detected by BrdU analysis. Data are shown as the mean ± SD, n = 4. *P < 0.05 by paired student’s t-test. (c) The expression levels of genes involved in cell proliferation were analysed by real-time PCR. Data are shown as the mean ± SEM, n = 4. **P < 0.01, ***P < 0.001 by paired student’s t-test. CTL siRNA: Control siRNA group.