Table 2. Primers used for cDNA amplification, dsRNA synthesis and qRT-PCR analysis.

Function	Primer	Sequence (5′–3′)
cDNA amplification	Mi-ace1-F	ATGATGGATTATTCAATAGAGGACAG
	Mi-ace1-R	CTATTTTATTCCACAAACATCATTATCACC
	Mi-ace2-F	GAGGTGAATTATGCGCAAACGAAG
	Mi-ace2-R	TTATTTGAAAATAAATGATGAATACAGGAAAGATATTTCAGG
dsRNA template synthesis	T7-iace2-F	TAATACGACTCACTATAGGGGATGGTAAATCTGTTCACAC
	T7-iace2-R	TAATACGACTCACTATAGGGGCACGCTCTTGAACCG
	T7-iegfp-F	TAATACGACTCACTATAGGGGAGTACAACTACAACAGCCAC
	T7-iegfp-R	TAATACGACTCACTATAGGGACGAACTCCAGCAGGACCAT
qRT-PCR	q-actin-F	GGGTATGGAATCTGCTGGTAT
	q-actin-R	AGAAAGGACAGTGTTGGCGTA
	q-ace2-F	GCTGGTGATGCTGTTTCTC
	q-ace2-R	CCACAAGAATGCTAACATCACG

The T7 sequences are underlined.