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. 2016 Nov 29;6:38115. doi: 10.1038/srep38115

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) Representative (left) and statistical (right) flow cytometric results of the Tfh cells differentiation. Naïve T cells were cultured under Tfh polarization condition, in the absence or presence of SM934 (10 μM) for 4 days. (B) Flow cytometric analysis of intracellular transcription factor Bcl-6. Shaded area depicted Th0 cells, full line represented Tfh cell culture, and dotted line showed Tfh cell cultured with DZ2002. (C) Bars showed mean fluorescence intensity (MFI) of Bcl-6 expression. Values are the mean ± SEM (n = 3). *p < 0.05 compared with the cell culture under Tfh differentiation condition. Results are derived from 3 independent experiments with similar results.