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. 2016 Nov 29;6:38115. doi: 10.1038/srep38115

Figure 6. SM934 inhibited generation of IL-21-producing T helper cells and impeded downstream signaling transduction.

Figure 6

(A) Representative (up) and statistical (low) results of the IL-21+ CD4+ T cell frequency in splenocytes from CIA mice determined by flow cytometry. (B) IL-21 mRNA level in splenocytes from CIA mice was measured by real-time PCR. Values are the mean ± SEM. Results are derived from 2 independent experiments with r pattern in each treated group (n = 8 in each group). (C) Western blot analysis of phosphorylated STAT-3 protein, in B cell-depleted splenoctys from C57BL/6 mice stimulated with exogenous IL-21 in the presence or absence of SM934 at indicated time points. Total STAT-3 and GAPDH proteins were used as the loading control. The full-length blots are included in the Supplementary Information 2. Representative blots from 3 experiments are showed in left panel. Right panel showed quantification of Western blotting images by ImageJ. Values are the mean ± SEM (n = 3). *p < 0.05, **p < 0.01 compared with the IL-21 treated cells in the absence of SM934. Results are derived from 3 independent experiments with similar results.