Table 3.
Potential DNA barcodes to identify Paris s.s. and Daiswa species.
| Loci | Location | Primers | GC % | Tm (°C) |
|---|---|---|---|---|
| ndhC/trnV-UAC | LSC | F:ACAAAACTTTCTCGCTCGGT R:TTCTATGGACCAAGCAACCG |
45.0 50.0 |
58.1 57.9 |
| trnN-GUU/ycf1 | IRB | F:CCGGAACTTCTTCGTAGTGG R:CCCCGAAGTGGCTCTATTTC |
55.0 55.0 |
58.0 58.0 |
| rps15/ycf1 | SSC | F:CATCTGGTATACGCAAAAGCG R:ACCTATGCGTACATCTTTCGG |
47.6 47.6 |
57.8 57.9 |
| rpl33/rps18 | LSC | F:AACAAAACGCGTGTTCGATC R:ATTTCGGCCGGATCTGAAAT |
45.0 45.0 |
58.0 57.7 |
| ndhA intron | SSC | F:ACCCATGTAATTCTGTCGGC R:GGGGAAGTACTGCTTGATCG |
50.0 55.0 |
58.0 58.1 |
| atpF intron | LSC | F:TTTGGCTCTCACGCTCAATT R:TCGCTTCGGCATTGGATAAA |
45.0 45.0 |
58.1 58.0 |
| psbZ/trnG-GCC | LSC | F:CCTCGATTCAAAAATGCCGT R:GCGAAAATATGATCCAGACGC |
45.0 47.6 |
57.1 57.8 |
| psaA/ycf3 | LSC | F:ACAAAGAGACCTGCCAACAG R:TGCAACCGAGTCCTAGTGTA |
50.0 50.0 |
58.0 58.1 |
| trnV-UAC intron | LSC | F:ACCTTGACTTAGGTCTGCCT R:CAAATCGATGGCGGGTTCTA |
50.0 50.0 |
58.0 58.1 |
| ccsA/ndhD | SSC | F:GGTTCTCAAAAACTCTAGAGGC R:TTGCATTCTACAGCGAACGA |
45.5 45.0 |
56.7 57.9 |