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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Virology. 2016 Sep 16;499:72–81. doi: 10.1016/j.virol.2016.09.007

Fig. 6.

Fig. 6

Knock-down of TLR4 rescues HIV expression. MDMs were transfected with siRNA against TLR4 or a non-targeting control. (A) Confirmed TLR4 down-regulation by RT-PCR 48 h post-transfection. Error bars represent the standard deviation of triplicate measurements. (B) Cells were infected with HIV-NL4-3ΔEnv-Luc(VSV-G) 72 h post-transfection under the conditions shown following the experiment outline in Fig. 1A and measured HIV expression by luciferase 3 days post-infection. Error bars represent the standard deviation of triplicate infections. (C) Engaging TLR2 with the agonist Pam3CSK4 (1 μg/ml) does not repress HIV expression to a similar extent as E. coli LPS (1 μg/ml). Error bars represent the standard deviation of infections done in triplicate and the data is representative of more than 3 independent experiments.