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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Plasmid. 2016 May 24;86:1–6. doi: 10.1016/j.plasmid.2016.05.001

Fig. 1.

Fig. 1

Visual representation of BspQI vector digestion. BspQI binds to the recognition site [GCTCTTC (1/4), highlighted in grey] and will cut both strands at indicated arrows. This creates a three base pair overhang. The cut site does not interfere with recognition and can be custom designed. Shown is an example of vectors constructed in this study (pCV1–3, pTEV18, 19). For a more detailed figure, see supplemental.