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. Author manuscript; available in PMC: 2017 Jul 1.
Published in final edited form as: Plasmid. 2016 May 24;86:1–6. doi: 10.1016/j.plasmid.2016.05.001

Fig. 2.

Fig. 2

Plasmid maps and MCSs of complementation vectors. Plasmid maps show notable genetic features. Plasmid MCS sequences of each vector are shown between NheI and HindIII restriction sites. S.D. in MCSs represents Shine-Dalgarno (ribosome-binding site) sequence. Asterisks identify location of BspQI sites in original vectors that were mutated from GCTCTTC to WCTWTTC, as explained in Materials and methods Section 2.3.