Fig. 3.
Growth analysis and complementation of S. enterica acs phenotype. S.e. acs+ was cloned into pCV1-pCV3 and transformed into a acs strain to assess the effectiveness of the vectors. Cells were grown in NCE medium with acetate (10 mM) and transcription of acs+ in each vector was induced with L(+)-arabinose (250 μM). Growth curves were obtained using a microplate reader (BioTek Instruments).