Table 1.
Vector | GenBank accession # | No. of positive clones (20 screened)a | Efficiency (%)b |
---|---|---|---|
pCV1 | KU974153 | 19 | 95 |
pCV2 | KU974154 | 20 | 100 |
pCV3 | KU974155 | 19 | 95 |
pTEV16 | KU974156 | 20 | 100 |
pTEV17 | KU974157 | 20 | 100 |
pTEV18 | KU974158 | 18 | 90 |
pTEV19 | KU974159 | 19 | 95 |
pTEV20 | KU974160 | 20 | 100 |
A positive result was indicated by a 2.2 kbband as shown in Fig. S2.
Colony PCR was used to screen 20 colonies for each vector.
Cloning efficiencies were calculated as described under Materials & methods Section 2.6.