Table 2.
Plasmid | Sequence to add to 5′ of primer |
---|---|
pTEV16 forward primer | 5′NNGCTCTTCNAGC |
pTEV17 forward primer | 5′NNGCTCTTCNACC |
pCV1, pCV2, pCV3, pTEV18, pTEV19 forward primer | 5′NNGCTCTTCNTTC |
pCV1, pCV2, pCV3, pTEV16, pTEV17, pTEV18, pTEV19 reverse primer | 5′NNGCTCTTCNTAA |
pTEV20 forward primer | 5′NNGCTCTTCNTAC |
pTEV20 reverse primer | 5′NNGCTCTTCNTTC |