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. 2016 Nov 28;13:195. doi: 10.1186/s12985-016-0650-z

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Frequency distribution of the negative sera tested using the FMDV/A cELISA. Serum samples were collected from disease–free bovine (n = 320), porcine (n = 475) and ovine (n = 379) in Canada. Rabbit polyclonal anti–FMDV/A serum pool was coated onto microtitre plates, thereafter BEI inactivated FMDV/A antigen was added. Equal volumes of diluted sera and mAbs (#5 and #7) were added to the plates and allowed to compete with antibodies in serum samples. Results are expressed as the percentage of inhibition