Fig. 1.

Scheme of computational prediction of small ORFs in cyanobacteria. Small ORFs were detected based on the transcriptome information for expressed intergenic regions [20–22] and their coding potential evaluated with RNAcode [40]. This information was merged with the pre-existing annotation [32]. Orthologs between the two small ORF populations were detected when they were identified as reciprocal best hits (RBH) by blastP with e ≤ 1e⁻²