Fig. 9.

Detection of μ-proteins upon expression from their native promoters and 5′ UTRs. Recombinant Synechocystis 6803 cells carrying the respective genes under control of their own promoters and 5′UTRs on vector pVZ322 were collected from cultures grown at standard conditions (0) and after transfer to the respective inducing condition at indicated time points (6 or 24 h) or in case of Ssr1169 after 24 h at standard condition. The Western blot was probed with specific ANTI-FLAG® M2-Peroxidase (HRP) antibody. All samples were separated on the same gel and transferred to the same membrane but the part probed for Norf4 had to be exposed longer because of its lower expression. Prestained Protein Ladder (10–170 kDa, Fermentas) was used as molecular weight marker