Table 2.

Sensitivity of mouse anti-SAM antibody 118-6

SAM standard (nM)	OD450	OD450	OD450	Mean	SD
Blank	0.0556	0.0808	0.0616	0.0660	0.0131
0	1.2189	1.2831	1.3030	1.2683	0.0439
6.3	1.110	1.0648	0.9757	1.0502	0.0683
12.5	0.885	0.8004	0.8737	0.8530	0.0459
25	0.6252	0.6435	0.6108	0.6265	0.0164
50	0.3838	0.3995	0.3782	0.3872	0.0110
Sensitivitya	2.6 nM

^aSensitivity was estimated by the corresponding concentration calculated from standard curve given the OD 450 (=OD450 at zero antigen – 2 × standard deviation of blank wells). ELISA was performed in triplicates. PLL-aza-SAM was coated at 0.1 µg/mL and the 118-6 used at 1:32,000