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. 2016 Nov 4;8(11):301. doi: 10.3390/v8110301

Figure 1.

Figure 1

Construction the recombinant fosmid with env and gag-IRES-env genes inserted at the US2 site in the Marek’s disease herpesviruses (MDV) genome. (A) the genomic structure of the MDV 814 strain; (B) the five fosmid DNAs used for MDV regeneration. Numbers show the fosmid names and the location of each fosmid fragment in the genome of the 814 strain; (C) the schematic diagrams of the mutant fosmid inserted with the Kan-ccdB dual selection markers flanked by the attR1 and attR2 sequences within the US2 gene; and (D) the schematic diagrams of recombinant fosmid inserted with the CAG-env or CAG-gag-IRES-env expression cassette within the US2 gene.