Figure 3.

Western blot and immunofluorescence analyses of the recombinant Env and Gag proteins expressed in the rMDV-infected CEFs. The primary antibodies used were gp85-specific mouse monoclonal antibody (Env) and p27-specific mouse monoclonal antibody (Gag). (A) Western blotting of recombinant MDVs expressing Gag and Env proteins from ALV-J. Chicken β-actin and MDV gI protein in the cell lysates was used as the internal control; and (B) detection of Env and Gag protein expression in the recombinant virus-infected CEFs using immunofluorescence.