Table 1.
Primers used for reverse transcription PCR (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR for ApGlV2, ALPV, and RhPV in A. glycines and for PCR to test for the presence of ApGlV2 sequences in the aphid genome. F denotes a forward primer while R denotes a reverse primer. N/A, not applicable.
| Primer Name | Sequence (5′–3′) | ApGlV2 Genome Position |
|---|---|---|
| Tet-F | AGTGGCTGCGCATGCTCGTT | 1695–1714 |
| Tet-R | ACGCGCCTCTCCGTTGAACT | 2604–2623 |
| ApGlV2 3′-F | CAGTACAGCAATACGGCTCATT | 4284–4305 |
| ApGlV2 3′-R | AAGGGCCTTATTACTTTTCACACTCTCTC | 4474–4502 |
| ApGlV2 5′-F | GCAGGACCTTGCCTCGCTCAAA | 160–181 |
| ApGlV2-RdRp-F | AGTGGCTGCGCATGCTCGTT | 1695–2014 |
| ApGlV2-RdRp-R | ACGCGCCTCTCCGTTGAACT | 2603–2623 |
| ApGlV2 R/C-F | CACGCGCGGAATCTTTGCAG | 2976–2995 |
| ApGlV2 R/C-R | TCGGTCTTGGCGGCGTCATA | 3825–3845 |
| ApGlV2-CP-F | AGCAGAGCTCAACACGACGAACCAAG | 3315–3329 |
| ApGlV2-CP-R | AGCAAGCTTCTAAGCTCTCGTG | 3953–3965 |
| ApGlV2 C/T-F | TGTGACTCCGACACCGTCGAA | 3885–3905 |
| ApGlV2 C/T-R | GCACCGGGAGAAATCCCAGAGT | 4424–4445 |
| ApGlV2 3′ RACE | TCTCCCGGTGCCTCGTCTCACCACAGG | 4435–4461 |
| ApGlV2-sgRNA 5′ RACE | CCCGAGAGTTTTATTTATGCTGGTGGACGATATGGGCAGAGACA | 3748–3791 |
| ApGlV2 5′ RACE | AAGTGCCGTAGCGCTGCCTCGAGCAC | 476–501 |
| ALPV-F | TGAACTTCGTGCAACGAACACTGTT | N/A |
| ALPV-R | TCCGCCTGCGTTAGGAAGAAGA | N/A |
| RhPV-F | AATCTGGCGTTGACGCGCTC | N/A |
| RhPV-R | TCCCCCATCATCAACATAGATGCGT | N/A |
| qRT-PCR ApGlV2-F | TCCCCGCCACGTGAAGTGAA | 2624–2643 |
| qRT-PCR ApGlV2-R | GCTACTGCGTGCGTGGTGAA | 2822–2841 |