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Detection of ALY- and Eph-mRNA in M. persicae (Sulzer) or in M. persicae ssp. nicotianae by reverse transcription polymerase chain reaction (RT-PCR; Supplementary Materials Table S1). PCR amplified fragments were loaded on a 1.5% agarose gel and visualized by UV after ethidium bromide staining. This detection is not quantitative since different amounts of RNA have been used in the RT-PCR reactions. T-: negative control, L: DNA ladder.
