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. 2016 Nov 28;9:260. doi: 10.1186/s13068-016-0674-z

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — Binding analysis of ChiA_M to insoluble polysaccharides. Lanes control, protein without substrate, U unbound protein, W wash fraction, E proteins eluted by incubation with urea. Lanes marked with an asterisk in the upper row refer to experiments using ball-milled chitin, while unmarked lanes refer to crushed chitin, sieved to a particle size of ≤0.85 mm