Table 1.
Biometric data
| Wt ND |
Wt HFD |
Epac1−/−
ND |
Epac1−/−
HFD |
Epac2−/−
ND |
Epac2−/−
HFD |
|
|---|---|---|---|---|---|---|
| N | 8 | 16 | 6 | 6 | 6 | 10 |
| BW (g) | 27.5 ± 1.6 | 44.5 ± 2.0& | 24.2 ± 0.5 | 48.4 ± 1.1& | 24.7 ± 0.6 | 38.8 ± 3.0& |
| HW (mg) | 146.3 ± 8.2¤ | 162.2 ± 6.7¤ | 154.3 ± 8.6¤ | 197.7 ± 6.0& | 126.5 ± 7.2¤ | 137.4 ± 5.3¤ |
| HW/BW (mg/g) | 5.3 ± 0.2€ | 3.7 ± 0.2& | 6.4 ± 0.4 | 4.1 ± 0.2& | 5.2 ± 0.3€ | 3.6 ± 0.2 & |
| LV volume (mm3) | 108.6 ± 6.7 | 117.5 ± 5.2 | 96.8 ± 4.8 | 124.7 ± 8.8 | 96.0 ± 5.4 | 104.9 ± 2.7 |
| Total fat (g) | 1.7 ± 0.3 | 7.7 ± 0.5& | 1.2 ± 0.1 | 8.6 ± 0.4& | 1.1 ± 0.1 | 6.9 ± 0.7&¤ |
| iWAT (g) | 0.4 ± 0.1 | 2.0 ± 0.1& | 0.3 ± 0.1 | 1.8 ± 0.1& | 0.4 ± 0.1 | 1.9 ± 0.2& |
| rWAT (g) | 0.5 ± 0.1 | 2.1 ± 0.2& | 0.3 ± 0.1 | 2.5 ± 0.2& | 0.3 ± 0.1 | 2.0 ± 0.2& |
| gWAT (g) | 0.8 ± 0.2 | 3.6 ± 0.3& | 0.6 ± 0.1 | 4.3 ± 0.2& | 0.5 ± 0.1 | 3.0 ± 0.3&¤ |
| rWAT + gWAT (g) | 1.3 ± 0.2 | 5.7 ± 0.4& | 0.9 ± 0.1 | 6.8 ± 0.3& | 0.8 ± 0.1 | 5.1 ± 0.5&¤ |
| Visceral fat (% of BW) | 4.6 ± 0.8 | 12.5 ± 0.5& | 3.4 ± 0.5 | 14.1 ± 0.4& | 3.4 ± 0.2 | 12.1 ± 0.7& |
| Body fat (% of BW) | 6.1 ± 1.0 | 17.0 ± 0.5& | 4.7 ± 0.4 | 17.8 ± 0.5& | 4.6 ± 0.4 | 16.7 ± 1.0&¤ |
Values represent means ± s.e.m
ND normal diet, HFD high fat diet, iWAT inguinal fat (subcutaneous fat), rWAT peri-renal fat, gWAT peri-gonadal fat, rWAT + gWAT visceral fat. & p < 0.05 vs. corresponding genotype on ND; € p < 0.05 vs. Epac1−/− ND; ¤ p < 0.05 vs. Epac1−/− HFD
Body and fat weights were measured at the end of the feeding protocol, while the heart weight was measured after the ex vivo perfusions. Left ventricular (LV) volume was determined during the infarct size assessment