Fig. 5.

GANT61 increased apoptosis of glioma cells treated with TMZ. Apoptosis of cells under different treatments were detected by flow cytometric analysis with Annexin V-FITC/PI staining. a U251 and (b) U87. Left: The representative quadrantal diagrams showing results of flow cytometric analysis. Right: Histogram manifested average apoptosis rates in cell groups of different treatments. GANT61 and TMZ treatment resulted in only a slight increase in apoptosis. *P < 0.05. Combined GANT61 and TMZ treatment resulted in a significant increase in early apoptosis rate compared to cells treated with GANT61 or TMZ alone, *P < 0.05, **P < 0.01. All data were representative of three independent experiments