Table 3.
Gene | Initial Denaturing | Denaturing | Annealing | Extension | Final Extension | Cycle |
---|---|---|---|---|---|---|
ITS (ITS5, ITS4) | 94 °C, 10 m | 94 °C, 30 s | 55 °C, 30 s | 72 °C, 1 m | 72 °C, 10 m | 30 |
EF (EF1, EF2) | 94 °C, 5 m | 94 °C, 30 s | 52 °C, 40 s | 72 °C, 1 m | 72 °C, 3 m | 35 |
EF (EF3, EF2T) | 94 °C, 5 m | 94 °C, 30 s | 53 °C, 30 s | 72 °C, 1 m | 72 °C, 5 m | 40 |
β-tubulin (BT2a, BT2b) | 94 °C, 5 m | 94 °C, 30 s | 60 °C, 30 s | 72 °C, 1 m | 72 °C, 3 m | 35 |