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. 2016 Oct 27;8(11):310. doi: 10.3390/toxins8110310

Table 3.

PCR condition for amplifying target genes used in this study.

Gene Initial Denaturing Denaturing Annealing Extension Final Extension Cycle
ITS (ITS5, ITS4) 94 °C, 10 m 94 °C, 30 s 55 °C, 30 s 72 °C, 1 m 72 °C, 10 m 30
EF (EF1, EF2) 94 °C, 5 m 94 °C, 30 s 52 °C, 40 s 72 °C, 1 m 72 °C, 3 m 35
EF (EF3, EF2T) 94 °C, 5 m 94 °C, 30 s 53 °C, 30 s 72 °C, 1 m 72 °C, 5 m 40
β-tubulin (BT2a, BT2b) 94 °C, 5 m 94 °C, 30 s 60 °C, 30 s 72 °C, 1 m 72 °C, 3 m 35