Figure 2.

Comet assay. Damage to the DNA of peripheral blood cells was observed under an Olympus fluorescence microscope (Olympus BX41, Olympus Corporation, Tokyo, Japan) equipped with an excitation filter of 515–560 nm and a barrier filter of 590 nm (400×). One hundred cells were examined from each broiler. (A) Class 0, control group fed soybean vehicle carrier only; (B) Class 1, control group with 60 mg·piperine·kg⁻¹ feed; (C) Class 2, group with piperine and 0.5 mg·AFB₁·kg⁻¹ body weight; (D) Class 3, group with aflatoxin B₁ only.