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The EC50 values for the analyzed toxins in the ADP-ribosylation assay. The 2-h EC50 values were determined in triplicates in solid-phase assay with S. cerevisiae eEF2 as a substrate. PE-inactive showed no ADP-ribosylation activity. PE-Sigma (Sigma Aldrich, St. Louis, MO, USA) was used as a control protein.
| Toxin | EC50 ± SD (μg/mL) |
|---|---|
| PE-native | 0.54 ± 0.01 |
| PE-inactive | - |
| PE-NLS | 0.57 ± 0.19 |
| PE-furin | 0.40 ± 0.08 |
| PE-Sigma | 3.00 ± 0.40 |
