Table 2.

PCR amplification protocol.

Gene	Profile Type	Initial Denaturing	Cycles	Denaturing Annealing Elongation	Final Elongation	Rest Period
ITS, BenA	standard	94 °C, 10 min	35	94 °C, 45 s; 55 °C, 45 s; 72 °C, 60 s	72 °C, 10 min	4 °C, ∞
RPB	touch-up	94 °C, 5 min	5	94 °C, 45 s; 50 °C, 45 s; 72 °C, 60 s	-	-
-	-	-	5	94 °C, 45 s; 52 °C, 45 s; 72 °C, 60 s	-	-
-	-	-	30	94 °C, 45 s; 55 °C, 45 s; 72 °C, 60 s	72 °C, 10 min	4 °C, ∞