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. 2016 Nov 19;8(11):341. doi: 10.3390/toxins8110341

Figure 3.

Figure 3

Adhesion of C. perfringens CN3718 to Caco-2 cells [34]. Caco-2 cells were incubated for 2 h at 37 °C under anaerobic conditions. C. perfringens CN3718 produces all three sialidases. This wild-type (WT) strain, and a complementing strain that produces only NanI, attach very well to Caco-2 cells as detected by immunofluorescence microscopy (600×). In comparison, only a few cells of an isogenic mutant with all three sialidase genes disrupted were able to attach to Caco-2 cells. Furthermore, when the triple mutant was complemented to produce only NanJ or NanH, those bacteria remained poorly adherent (not shown). Green: C. perfringens; Red: Caco-2 cells. Reproduced with permission from [34]. Creative Commons License 2011, Copyright J. Li and B.A. McClane.