Table 1. GenBank accession numbers, primer sequences and amplicon sizes of the genes analyzed in this study.
| Gene | Accession number | Primer sequences (5’ → 3’) | Amplicon size1 |
|---|---|---|---|
| CHD8 | XM_003482263.1 | F: AGTGAGGACGAGAAGGAAGA | 104 |
| R: GGGAATCCATCTTGGGACATAG | |||
| CSRP3 | NM_001172368.1 | F: CAGCAACCCTTCCAAGTTCA | 91 |
| R: CATCACCTTCTCAGCAGCATAG | |||
| EID2B | XM_003127131.1 | F: CGCCACTATCTGGAACACTAC | 122 |
| R: CGCTGATATTCGGCATCAAAC | |||
| HIF1AN | XM_003359328.1 | F: GTACTGGTGGCATCACATAGAG | 118 |
| R: CTGATGGGCTTTGAGAGGATATT | |||
| IKBKB | NM_001099935.1 | F: GATGGCGACAGTCAGGAAAT | 107 |
| R: TTGCAAACCACCGTCTTACT | |||
| LEF1 | NM_001129967.1 | F: CTATTGTAACGCCTCAGGTCAA | 99 |
| R: TTGGCTCTTGCTCCTTTCTC | |||
| MAP2K1 | NM_001143716.1 | F: GGAGCTGGAGCTGATGTTT | 110 |
| R: GTCGGCTGTCCATTCCATAA | |||
| MRAS | XM_003358570.2 | F: GGTCGATTTGATGCATTTGAGG | 96 |
| R: TCCTTGGCACTGGTTTCTATG | |||
| MYOG | NM_001012406.1 | F: CAGGCTCAAGAAGGTGAATGA | 118 |
| R: GCACTCGATGTACTGGATGG | |||
| RBM24 | XM_001925447.3 | F: TACCTGCCCACTATGTCTATCC | 118 |
| R: GCAGCTCCCGTGTAATCAAT | |||
| RSPO3 | NM_032784.4 | F: GAAACACGGGTCCGAGAAATA | 110 |
| R: CCCTTCTGACACTTCTTCCTTT | |||
| SOX7 | XM_003359052.1 | F: TCTCCACTCCAACCTCCA | 120 |
| R: TCATTGCGATCCATGTCCTC | |||
| SUFU | XM_001928912.4 | F: GGAGCCCTCATTCCTCTTTG | 83 |
| R: GCCATGTCACCTGTGATACTT | |||
| ACTB2 | XM_003124280.3 | F: AAGATCAAGATCATCGCGCCTCCA | 108 |
| R: ACTCCTGCTTGCTGATCCACATCT | |||
| GAPDH2 | NM_001206359.1 | F: ACAGTCTTCTGGGTGGCAGTGAT | 176 |
| R: CATGTTTGTGATGGGCGTGAACAA | |||
| HPRT12 | NM_001032376.2 | F: GCTGACCTGCTGGATTACAT | 101 |
| R: CTGGTCATTACAGTAGCTCTTCAG |
Amplicon size in nucleotide number,
Reference gene. CHD8 – chromodomain helicase DNA binding protein 8, CSRP3 – cysteine and glycine-rich protein 3, EID2B – EP300 interacting inhibitor of differentiation 2B, HIF1AN – hypoxia inducible factor 1, α subunit inhibitor, IKBKB - inhibitor of κ light polypeptide gene enhancer in B-cells, kinase β, LEF1 – lymphoid enhancer-binding factor 1, MAP2K1 – mitogen-activated protein kinase kinase 1, MRAS – muscle RAS oncogene homolog, MYOG – myogenin (myogenic factor 4), RBM24 – RNA binding motif protein 24, RSPO3 – R-spondin 3, SOX7 – SRY (sex determining region Y)-box 7, SUFU – suppressor of fused homolog, ACTB – β-actin, GAPDH – glyceraldehyde-3-phosphate dehydrogenase, HPRT1 – hypoxanthine phosphoribosyltransferase 1.