Figure 5. Expression profiles of the DGAT1, DGAT2, DGAT3 and WS/DGAT genes during soybean seed development using RT-qPCR analysis. Expression profiles of each DGAT3 (Glyma13g17860, Glyma17g04650), WS/DGAT (Glyma09g32890), DGAT1 (Glyma13g16560, Glyma17g06120) and DGAT2 (Glyma01g36010, Glyma09g32790, Glyma11g09410, Glyma16g21960, Glyma16g21970) genes. The comparison of expression profiles between DGAT3 and WS/DGAT genes was done using an endogenous normalization with the leaf tissue of WS/DGAT gene. Standard error bars are based on four biological replicates. One-way ANOVA followed by the Tukey's test was used in the statistical analysis of the RT-qPCR data. mRNA input normalization was performed with four constitutive genes (Actin, ELF, CPY and TUA).
