Figure 1.

EGFP transfection fold‐changes versus Hoechst‐count fold‐changes are shown for (A) 369 hits with fold‐increases in transfection at 5 µM, (B) 72 hits with fold‐decreases in transfection at 5 µM, (C) 201 hits with fold‐increases in transfection at 50µM, and (D) 132 hits with fold‐decreases in transfection at 50 µM. To examine effect of transfection priming on cell proliferation, linear regressions were performed, demonstrating negative correlation for transfection fold‐increases (AC) and no correlation for transfection fold‐decreases (BD). Regression slopes are displayed as the 95% confidence interval, along with R ² for goodness of fit. The dashed lines indicate the Hoechst‐count fold‐change threshold of greater than 0.5 used in determining the largest transfection fold‐increases and fold‐decreases of hits in the screen (Tables 1 and 2).