Table 2.

Highest fold‐changes in transfection priming hits at 50 mM of NCC compounds

	Compound	Drug type	Transfectiona	Cell‐countb	Hoechst‐intensityc	Resazurin‐intensityd
Transfection fold‐increase	Tranilast	Anti‐allergy	3.63	0.55		1.24
	Piceid	Stilbenoid	2.63	0.69	1.17
	5‐Fluorocytosine	Pyrimidine analogue	2.54	0.52	1.50	1.58
	Cinanserin	Serotonin receptor antagonist	2.53	0.63	1.41
	Zardaverine	Phosphodiesterase inhibitor	2.26	0.55	1.36
	Nateglinide	ATP potassium channel closer	2.08	0.51	1.23	1.21
	Eryped	Macrolide antibiotic	1.99	0.72	1.26	1.26
	Mestinon	Cholinesterase inhibitor	1.83	0.77	1.21	1.23
	Acyclovir	DNA polymerase inhibition (anti‐viral)	1.78	0.60
	Stiripentol	GABA receptor modulator	1.77	0.72	1.23
Transfection fold‐decrease	Epigallocatechin gallate	Flavonoid	0	0.80
	Cefixime trihydrate	β‐lactam antibiotic	0.017	1.45	0.84
	Cefdinir	β‐lactam antibiotic	0.032	1.35	0.88
	Cefuroxime	β‐lactam antibiotic	0.039	1.39
	Rolitetracycline	Tetracycline antibiotic	0.063	1.42	0.86
	Cefatrizine propylene glycol	β‐lactam antibiotic	0.095	1.75	0.78
	Tetracycline	Tetracycline antibiotic	0.139	1.08	0.85
	Taxifolin‐(+)	Flavonoid	0.162	1.22	0.87
	(+/−)‐Epinephrine hydrochloride	Adrenergic receptor agonist	0.35	1.36	0.83
	IsoquercitrinHyperoside	FlavonoidFlavonoid	0.358	1.68	0.86

Note. Empty values indicate nonsignificant fold‐change.

^aTransfection fold‐changes were calculated from duplicate averages of EGFP fluorescence, measured by EGFP cell‐count (image processing) or EGFP intensity (plate reader and image processing), relative to the same measurement averaged from the vehicle controls in each compound's respective plates. These measurements were normalized in two separate ways, total cell‐count (determined by Hoechst‐count), and transfected cell‐count (determined by EGFP‐count), for measurement of transfection per cell as well as transfection per transfected cell.

^bCell‐count fold‐changes were calculated from duplicate averages of image processing measurements of Hoechst‐count relative to the average Hoechst‐count measurements of vehicle controls in each compound's respective plates. Cell‐count fold‐changes are shown for each hit presented as a general toxicity reference, not to imply significant increase or decrease.

^cHoechst‐intensity fold changes were calculated from duplicate averages of plate reader or image processing measurements of Hoechst‐intensity normalized by total cell‐count (determined by Hoechst‐count), relative to the average Hoechst‐intensity measurements of vehicle controls in each compound's respective plates.

^dResazurin‐intensity fold changes were calculated from duplicate averages of plate reader measurements of resazurin intensity normalized by cell‐count (determined by Hoechst‐count), relative to the average resazurin‐intensity measurements of vehicle controls in each compound's respective plates.