Table 3.

Average fold‐changes^a of transfection priming hits from clusters of interest (Figure 2)

		Transfection fold‐increase hits			Transfection fold‐decrease hits
Cluster (#)	Concentration	# of hits	Transfectionb	Cell‐countc	# of hits	Transfectionb	Cell‐countc
Hormones (54)	5 µM	34*	1.43 ± 0.22	0.73 ± 0.15	3	0.81 ± 0.07	0.93 ± 0.19
	50 µM	15	1.35 ± 0.16	0.66 ± 0.86	9	0.74 ± 0.12	0.84 ± 0.24
Glucocorticoids (30)	5 µM	17*	1.37 ± 0.14	0.77 ± 0.14	3	0.81 ± 0.07	0.93 ± 0.19
	50 µM	12	1.37 ± 0.15	0.67 ± 0.10	3	0.81 ± 0.04	1.01 ± 0.08
Tetracyclines (6)	5 µM	5*	1.27 ± 0.15	0.82 ± 0.06	1	0.91	0.72
	50 µM	0			3*	0.26 ± 0.27	1.07 ± 0.36
Fluoroquinolones (8)	5 µM	5*	1.54 ± 0.24	0.64 ± 0.17	0
	50 µM	4	1.56 ± 0.25	0.63 ± 0.11	2	0.65 ± 0.21	0.96 ± 0.09
β‐lactams (18)	5 µM	7	1.25 ± 0.17	0.93 ± 0.13	2	0.69 ± 0.12	1.26 ± 0.14
	50 µM	5	1.25 ± 0.11	0.96 ± 0.20	9*	0.42 ± 0.37	1.31 ± 0.21
Cephalosporins (10)	5 µM	4	1.33 ± 0.19	0.85 ± 0.09	1	0.61	1.36
	50 µM	1	1.19	1.08	7*	0.31 ± 0.35	1.35 ± 0.22
Macrolides (4)	5 µM	3*	1.24 ± 0.05	0.82 ± 0.19	0
	50 µM	2*	1.72 ± 0.39	0.63 ± 0.12	2*	0.74 ± 0.18	0.85 ± 0.42
Flavonoids and Stilbenoids (13)	5 µM	3	2.1 ± 0.86	0.72 ± 0.37	4	0.52 ± 0.33	1.04 ± 0.51
	50 µM	3	2.04 ± 0.61	0.69 ± 0.09	4	0.32 ± 0.32	1.26 ± 0.36
GABA modulators (9)	5 µM	7*	1.74 ± 0.63	0.66 ± 0.14	0
	50 µM	4	1.41 ± 0.25	0.72 ± 0.04	0

Note. Nobileton, a flavonoid (Table 1), was not automatically grouped into the flavonoid and stilbenoid cluster (Figure 2) by the clustering algorithm; it was manually added to the flavonoid and stilbenoid cluster for the calculation of cluster average fold‐changes. Similarly, stiripentol (Table 2) and zolpidem (Table 1) are GABAA receptor modulators which were not automatically clustered with the benzodiazepines (Figure 2); their fold‐changes were included into the GABAA modulator cluster for calculation of cluster average fold‐changes (Table 3).

^aAverage fold‐changes are shown as mean ± standard deviation.

^bTransfection fold‐changes of hits from the same cluster were averaged. The transfection fold‐changes of hits were calculated from duplicate averages of EGFP fluorescence, measured by EGFP cell‐count (image processing) or EGFP intensity (plate reader and image processing), relative to the same measurement averaged from the vehicle controls in each compound's respective plates. These measurements were normalized in two separate ways, total cell‐count (determined by Hoechst‐count) and transfected cell‐count (determined by EGFP‐count), for measurement of transfection per cell as well as transfection per transfected cell.

^cCell‐count fold‐changes of hits from the same cluster were averaged. The cell‐count fold‐changes were calculated from duplicate averages of image processing measurements of Hoechst‐count relative to the average Hoechst‐count measurements of vehicle controls in each compound's respective plates. Average cell‐count fold‐changes are shown for clustered hits as a general toxicity reference, not to imply significant increase or decrease.

*The majority of compounds in the cluster were hits for this fold‐change.

^#Denotes the number of NCC compounds in the cluster.