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. 2016 Sep 27;65(12):3585–3597. doi: 10.2337/db16-0709

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© 2016 by the American Diabetes Association.

Readers may use this article as long as the work is properly cited, the use is educational and not for profit, and the work is not altered. More information is available at http://www.diabetesjournals.org/content/license.

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Skeletal muscle (A) glycogen and amino acids (B) Ala, Gly, and Glu/Gln in fasted (Fa) and fed NT and hGLUT4 TG mice fed a CD (Lean) or an HFD (Obese) for 5 weeks. Data are the mean and SEM for five animals per group. Data were analyzed by two-way ANOVA. Pairwise comparisons of 95% CIs established significant differences (P < 0.05) between groups. The letter “a” designates a significant difference due to nutritional state (fasted or fed) for a given strain (NT or TG) or body mass (Lean or Obese). The letter “b” designates a significant different due to strain for a given nutritional state or body mass. The letter “c” indicates a significant difference due to body mass for a given nutritional state or strain. C: Representative Western blots (n-3 independent sets) imunoblotted (IB) for GLUT4 and slc38a2 levels from whole-cell skeletal muscle extracts.